We've built a new version of this page with an interactive 3D viewer. Try it out in beta and help us improve:

› 8xyx

Electron Microscopy
2.8Å resolution

Cryo-EM structure of SAM-bound Tetrahymena DNA methyltransferase complex MTA1c (D209A)

Released:
DOI: 10.2210/pdb8xyx/pdb
PDB entry 8xyx coloured by chain, front view.
PDB entry 8xyx coloured by chain, side view.
PDB entry 8xyx coloured by chain, top view.
Source organism: Tetrahymena thermophila SB210
Entry authors: Xu Q, Shi ZB
Related structures: EMD-38782

Function and Biology Details

Reaction catalysed: 
[an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosinein mRNA + S-adenosyl-L-homocysteine + H(+).]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-308027 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
mRNA m(6)A methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 43.32 KDa
Source organism: Tetrahymena thermophila SB210
Expression system: Escherichia coli
UniProt:
  • Canonical: Q22GC0 (Residues: 57-428; Coverage: 87%)
Gene name: TTHERM_00704040
Sequence domains: MT-A70
Methyltransferase MT, putative Chain: B
Molecule details ›
Chain: B
Length: 357 amino acids
Theoretical weight: 41.27 KDa
Source organism: Tetrahymena thermophila SB210
Expression system: Escherichia coli
UniProt:
  • Canonical: Q22XT1 (Residues: 1-324; Coverage: 100%)
Gene name: TTHERM_01005150
Myb-like domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 364 amino acids
Theoretical weight: 41.94 KDa
Source organism: Tetrahymena thermophila SB210
Expression system: Escherichia coli
UniProt:
  • Canonical: Q22VV9 (Residues: 2-360; Coverage: 100%)
Gene name: TTHERM_00161750
Sequence domains: Myb-like DNA-binding domain
Transmembrane protein, putative Chain: D
Molecule details ›
Chain: D
Length: 146 amino acids
Theoretical weight: 17.04 KDa
Source organism: Tetrahymena thermophila SB210
Expression system: Escherichia coli
UniProt:
  • Canonical: I7M8B9 (Residues: 154-295; Coverage: 48%)
Gene name: TTHERM_00439330

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-38782
Expression system: Escherichia coli

Quick links